FastQCFastQC Report
Wed 25 May 2016
SRR1700321_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700321_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12092897
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA276970.2290352758317548No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175610.14521747766478124No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130280.10773266323197825No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA126320.10445801365876184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG117650.024.909842
GTACCTG120400.024.796871
ACCTGGG125150.022.858783
CCTGGGG101250.017.2190074
TATAACG7950.016.1507742
GTACACG9400.015.5926851
ATAACGC9200.015.4252753
TAACGCA8950.014.597124
GTACCCG12550.014.0147781
GTATCAA397850.014.0051821
GTACATG280800.013.9488391
TACCCGG11650.013.728342
CGCGTAT1000.007567248313.49709412
TACATGG281900.013.2807172
TAGACCG2805.875336E-1012.8545885
ACATGGG283300.012.6344843
AGAGTAC396150.012.45303411
TACACGG10450.012.2869522
GTACCGG11700.012.1419951
GAGTACT253150.012.0939812
Produced by FastQC (version 0.11.5)