FastQCFastQC Report
Wed 25 May 2016
SRR1700320_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700320_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10588118
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146100.13798486189897013No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112330.10609061969275371No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG120950.027.2920511
TACCTGG121400.026.6434752
ACCTGGG133200.023.792323
ATAACGC8700.022.7829693
TATAACG8850.021.8882142
GTACCGG10700.021.0725541
TAACGCA8750.020.3353464
CCTGGGG90800.020.2660314
GGTATCA149800.019.5824221
GTATCAA255050.018.6092011
TACCGGG11200.017.4967082
GTACCCG11200.016.1054531
TACCCGG10800.015.8505742
GTACACG7550.015.828141
ATCAACG313800.014.8222473
TCAACGC316500.014.7095474
TATCAAC321750.014.58229352
CTGGGGG49700.014.4841355
CAACGCA321900.014.4380435
AACGCAG332350.014.1600566
Produced by FastQC (version 0.11.5)