FastQCFastQC Report
Wed 25 May 2016
SRR1700319_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700319_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14793017
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA201480.13619939732375078No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193090.1305278024083931No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG100100.017.0284061
TACCTGG97400.016.7657952
GTATCAA404400.015.6556911
GGTATCA265800.014.8531111
ACCTGGG112400.014.047383
GTACATG375800.013.7333131
TACATGG377650.013.1809862
TATAACG8900.013.1600772
ATCAACG480900.012.85675053
TCAACGC482250.012.8482184
ACATGGG379700.012.72419553
GTATTAG37250.012.7109881
CAACGCA488400.012.694115
TATCAAC493450.012.6302262
AACGCAG497350.012.5425686
ATAACGC9750.012.4747783
AGAGTAC460300.012.10552811
GAGTACT276100.012.07461212
TATACTG52900.012.0345395
TAACGCA8500.011.9238954
Produced by FastQC (version 0.11.5)