FastQCFastQC Report
Wed 25 May 2016
SRR1700318_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700318_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9874262
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112350.11378065520238373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG75750.023.0970671
TACCTGG77750.022.2780082
ACCTGGG86550.019.4920963
GGTATCA144350.017.8684541
GTACCGG6650.017.2912481
GTATCAA235950.017.1045191
CCTGGGG63800.014.8272014
TACACGG6750.014.34878352
ATAACGC6000.014.2651433
ATCAACG283100.013.9471623
TCAACGC284400.013.9225854
CAACGCA289250.013.6647945
TATCAAC293100.013.6021592
GTACATG209450.013.5418511
AACGCAG298850.013.4516626
TACCGGG7500.013.2142282
TATAACG6500.013.1680242
GTACACG7200.013.1520841
TACATGG205500.013.1418882
TAACGCA6750.012.6797424
Produced by FastQC (version 0.11.5)