FastQCFastQC Report
Wed 25 May 2016
SRR1700317_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700317_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9394456
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135300.14402111202607154No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101310.10784019851708285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG113800.027.7767031
TACCTGG112150.027.6353972
ACCTGGG120850.024.8628623
TATAACG7500.024.62632
GGTATCA110200.022.3621461
ATAACGC9050.021.1549633
TAACGCA9050.021.1542874
GTATCAA202050.020.5098081
CCTGGGG92450.019.6848684
GTACACG8400.017.9833451
GTACCGG9550.017.2343331
TACCGGG9200.016.4033892
ATCAACG248100.016.2505553
GTACCCG10000.016.2333241
TCAACGC248850.016.1739084
GTATAAC19650.016.0635091
CAACGCA253600.015.9138015
TATCAAC258050.015.7637832
AACGCAG264850.015.37373356
TACACGG8950.014.848262
Produced by FastQC (version 0.11.5)