FastQCFastQC Report
Wed 25 May 2016
SRR1700316_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700316_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10357434
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA210430.2031680819786059No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152120.14687035418231967No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114360.11041344796404205No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG90600.022.5899281
TACCTGG88300.022.4959282
ACCTGGG97050.019.9096013
ATAACGC7050.016.9300253
TATAACG6800.016.8910752
TAACGCA7300.015.4247444
CCTGGGG77900.015.4085084
GTACCGG8450.014.9378651
GTATCAA329450.014.7918821
GTACATG251550.013.2436391
AGAGTAC340400.012.56918711
TACATGG254400.012.4469872
GTATTAG27150.012.3701121
ATCAACG389000.012.31370453
TCAACGC390350.012.2653494
GAGTACT209300.012.25230112
GGTATCA223800.012.1060261
CAACGCA396600.012.0754395
TATCAAC399850.012.0140592
TACCCGG9200.011.99511052
Produced by FastQC (version 0.11.5)