FastQCFastQC Report
Wed 25 May 2016
SRR1700315_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700315_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11118118
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152770.1374063488083145No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA141620.12737767309179485No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114340.10284114631631No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG82300.020.4322151
TACCTGG83150.019.6914142
ACCTGGG90250.017.567373
TATAACG6750.017.0165352
GTATCAA301750.016.8526541
ATAACGC7550.015.809213
TAACGCA7300.015.7336184
GTACCGG9500.015.1856291
GGTATCA190750.015.1495561
TAGACCG2306.71389E-913.69238955
ATCAACG364900.013.6210233
TCAACGC365650.013.6054034
CAACGCA371500.013.3817385
TATCAAC375500.013.3631852
AACGCAG381800.013.0914336
GTACCCG8000.012.9611731
CGCGACT1751.2729988E-512.85402217
AGAGTAC355200.012.70395611
CCTGGGG70900.012.6420674
TACCGGG9100.012.6221552
Produced by FastQC (version 0.11.5)