FastQCFastQC Report
Wed 25 May 2016
SRR1700314_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700314_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10083853
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145940.14472642550421946No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113840.11289335534740541No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA127300.022.8612351
GTACCTG85400.020.5891571
TACCTGG83800.020.3991152
GTATCAA231450.019.7910271
ACCTGGG92850.018.0464463
TCAACGC287750.015.5831974
TATAACG6650.015.5793652
ATCAACG289400.015.5099153
CAACGCA292250.015.3709455
TATCAAC296850.015.21974852
AACGCAG303700.014.89520556
GTCGTAC2801.8189894E-1214.4915991
ATAACGC8400.014.2101033
TAACGCA7250.013.9789694
CCTGGGG78500.013.7712144
GTACATG251850.013.4082371
ACGCAGA351250.012.8277447
TATACTG36850.012.7580955
TACATGG253100.012.6893972
CGCAGAG358800.012.5828958
Produced by FastQC (version 0.11.5)