FastQCFastQC Report
Wed 25 May 2016
SRR1700313_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700313_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10471886
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110660.10567341928664999No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG76300.020.2472322
GTACCTG78300.020.2085911
GGTATCA145200.017.8366851
GTATCAA235750.017.5733471
ACCTGGG87550.017.0012253
TATAACG6800.014.2405122
ATCAACG288050.014.048023
TCAACGC288650.013.9876154
CAACGCA293150.013.7893235
TAGACCG2804.0017767E-1113.65763955
TATCAAC298900.013.6445312
AACGCAG302950.013.4175756
TACCCGG8650.013.2776172
CGCGTAT1902.0708703E-613.02350712
GTACATG227100.012.9028871
TATACTG36150.012.632015
TAACGCA7000.012.5458914
CCTGGGG67400.012.4952994
GTACCCG9700.012.3158631
TACATGG226700.012.2682192
Produced by FastQC (version 0.11.5)