FastQCFastQC Report
Wed 25 May 2016
SRR1700312_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700312_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9083722
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134390.14794596311952302No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103320.11374192208876494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG84200.025.2465131
TACCTGG83900.024.6141832
TATAACG6600.022.179282
GGTATCA117800.022.0640831
ACCTGGG94350.021.4095253
TAACGCA7050.020.4431274
ATAACGC7400.020.389173
GTATCAA212900.019.8318821
CCTGGGG72150.016.1677634
TCAACGC264650.015.5631774
ATCAACG263950.015.5617933
GTACACG7150.015.4486991
CAACGCA269950.015.2991545
TATCAAC273250.015.1235572
AACGCAG279900.014.8356626
GTACCGG8800.014.345221
TACCGGG8250.013.9217632
TAGACCG2154.094909E-813.601385
TACCCGG7800.013.281322
TACACGG7150.013.2288262
Produced by FastQC (version 0.11.5)