FastQCFastQC Report
Wed 25 May 2016
SRR1700311_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700311_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9589198
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156430.1631314735601455No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123580.12887417696453865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG71750.019.5558642
GGTATCA154800.019.4098241
GTACCTG76950.018.8056721
GTATCAA261100.018.5865861
ACCTGGG76450.017.8521353
TATAACG6750.015.0148032
ATAACGC6450.015.0142543
ATCAACG321600.014.811173
TCAACGC323450.014.7055654
TATCAAC329400.014.55672
CAACGCA327650.014.5273435
TAACGCA7100.014.2741144
AACGCAG336950.014.2465486
CGAACCG1150.001205036613.6924435
GTACCGG6850.013.4913611
GTACATG224100.013.3271251
GTACCCG8650.013.0291591
CTAATAC25050.012.9464223
TACCCGG7850.012.9108172
GAGTACT228950.012.88113212
Produced by FastQC (version 0.11.5)