FastQCFastQC Report
Wed 25 May 2016
SRR1700310_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700310_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7338451
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127960.1743692231507712No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96100.13095406646443508No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG96950.028.4109251
TACCTGG95850.028.219052
TATAACG7450.025.9976252
TAACGCA7300.025.9142744
ACCTGGG102050.025.908583
ATAACGC8500.023.0509533
CCTGGGG72200.020.493254
GTACCCG9850.019.9087961
GGTATCA113000.019.7079051
TACACGG5150.019.2414132
GTACCGG6650.018.9814051
TACCCGG10000.018.9178072
GTATCAA194900.018.793321
GTACACG6600.016.3930321
GTATAAC17100.015.8178381
ACAACGC3700.015.2169233
ATCAACG238650.015.0045943
CTGGGGG36000.014.9977665
TCAACGC240550.014.8765144
TATCAAC243700.014.786182
Produced by FastQC (version 0.11.5)