FastQCFastQC Report
Wed 25 May 2016
SRR1700309_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700309_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11566896
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA669190.5785389615329817No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA314400.2718101727550762No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT224750.19430450485592676No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT209340.18098200243176735No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC179080.15482113784026416No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159430.1378330020430719No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA158210.13677826791215206No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT155510.13444402024536228No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA134220.11603804512463846No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121190.10477313879194558No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGT116690.10088272601396261No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG73050.016.6344321
TACCTGG71950.016.279452
GTACATG306050.013.9295991
ACCTGGG82000.013.8719983
TACATGG307300.013.4065892
TATAACG5650.013.1562642
GAGTACT226400.012.94673512
ACATGGG311300.012.7131793
AGAGTAC385000.012.60928511
ACCGTCC4300.012.5557488
AGTACTT233550.012.3577413
CCGACGC5300.012.30940132
TCTATAC24350.012.118083
GTACCGT4650.012.094476
GTATTAG25350.011.8280511
GTACTTT245800.011.63204114
TATACTG35450.011.6127385
GTATAAG29750.011.5185041
ACACCGT9200.011.4923766
TAACGCA5800.011.2621214
Produced by FastQC (version 0.11.5)