FastQCFastQC Report
Wed 25 May 2016
SRR1700308_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700308_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8017132
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105980.1321919110225452No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83500.10415195858069944No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG81350.024.9672451
TACCTGG81300.024.3216112
ACCTGGG86850.021.963383
GGTATCA117100.019.1736971
CCTGGGG67600.017.8897844
GTATCAA196600.017.7840921
TATAACG5800.017.084922
ATAACGC5700.016.9894473
TAACGCA6000.016.5150134
TACCCGG9050.015.4287752
GTACCCG9400.014.8685031
ATCAACG236200.014.4736323
TCAACGC238650.014.3719584
CAACGCA243800.014.0993245
TATCAAC245750.014.02123452
TACACCG4850.013.9151595
AACGCAG251200.013.7466666
ACCGTCC2957.2759576E-1213.7264798
GTCGTAC2358.811185E-913.4296161
GTACACG6250.012.984521
Produced by FastQC (version 0.11.5)