FastQCFastQC Report
Wed 25 May 2016
SRR1700307_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700307_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11368193
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA237800.20918012211791265No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144330.12695949127535044No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG98050.023.846512
GTACCTG101500.023.3260081
ACCTGGG109350.020.743683
TACCCGG10900.015.9125332
GTACCCG11650.015.8710281
TATAACG7800.015.59462
CCTGGGG87400.015.1538764
ATAACGC8700.014.7581083
GTACATG267100.014.2331441
GTACCGG9200.014.2153411
GTATCAA355850.013.7375551
TACATGG262800.013.6798912
GTCGTAC3500.013.5290851
TATACTG40850.013.4918595
TAACGCA8100.013.3479364
ACATGGG266800.012.9260093
TCTATAC30750.012.6729023
TACACGG8950.012.5840972
GTATTAG34850.012.42268851
AGAGTAC351050.012.14333711
Produced by FastQC (version 0.11.5)