FastQCFastQC Report
Wed 25 May 2016
SRR1700306_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700306_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7871137
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91210.11587906550222667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG64750.022.6362231
TACCTGG63500.022.2759062
GGTATCA97150.021.678761
ACCTGGG69700.019.7773513
GTATCAA168850.019.3507461
GTACCGG5300.016.5927771
ATCAACG212200.014.9241443
TCAACGC213000.014.8891454
TATAACG4550.014.8511242
CAACGCA216350.014.6921515
TATCAAC220650.014.5465632
ATAACGC4200.014.4798473
AACGCAG223050.014.3819386
CCTGGGG55650.014.2874994
TAGACCG2254.9258233E-913.9973445
TATACTG29500.013.9549785
TACCCGG6150.013.9173952
GTACATG191750.013.8881661
GTACCCG6500.013.8764061
TACATGG190150.013.3972522
Produced by FastQC (version 0.11.5)