FastQCFastQC Report
Wed 25 May 2016
SRR1700305_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700305_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7047973
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104300.14798581095585922No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78060.11075524835296616No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA73750.10464001493762816No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG93050.029.6543881
TACCTGG92850.029.0350512
ATAACGC6500.027.0264233
ACCTGGG101150.026.1847673
TATAACG6900.024.154222
TAACGCA6900.024.1535344
CCTGGGG70250.022.3131544
GTACCGG7050.021.4244461
GTACCCG8300.020.0991441
TACCCGG8450.019.1904952
GGTATCA115100.018.1171681
GTATCAA191850.018.1077631
TACCGGG7150.017.9547422
GTACACG8100.015.5857231
CTGGGGG35800.015.2698315
GTATAAC17400.015.0290891
TCAACGC228900.014.8864524
ATCAACG228300.014.8568373
GTCTCGC9150.014.7827111
CAACGCA233550.014.6218685
Produced by FastQC (version 0.11.5)