FastQCFastQC Report
Wed 25 May 2016
SRR1700304_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700304_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6571745
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA119980.18256946975270646No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80960.12319406793781561No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG75550.028.8537751
TACCTGG75350.028.5443652
ATAACGC6350.026.2439043
GTACCCG4900.026.2234251
TATAACG6700.026.2192362
GTACCGG5900.025.2175311
ACCTGGG85100.024.7959353
TACCGGG6000.024.3984582
TAACGCA6750.024.3550824
TACCCGG5450.022.3150312
CCTGGGG50500.020.5133654
GTATAAC17300.015.8973381
GTCTCGC9000.015.5295841
ACCGGGG4400.015.354623
TCTGTCG10000.015.0717128
GTATCAA203450.014.9362951
ACCGGGA4050.014.4573543
CTGGGGG26900.014.216235
GTACACG4600.014.2118581
TACACGG4650.014.0457612
Produced by FastQC (version 0.11.5)