FastQCFastQC Report
Wed 25 May 2016
SRR1700303_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700303_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6034370
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA83220.13791000551838883No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82890.1373631381569244No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65910.10922432664884653No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG75800.028.7878071
TACCTGG74250.028.6346762
ATAACGC5750.026.6337663
ACCTGGG79950.025.8592383
TATAACG5450.025.208682
TAACGCA6500.023.214164
GTACCCG7150.021.439041
CCTGGGG56350.020.5828174
TACCCGG7700.019.0125062
GTACCGG5000.018.9357171
GTACACG5200.017.7739091
TACACGG5200.016.025632
GTATCAA176600.015.2027981
GTATAAC14200.015.0812631
GGTATCA117900.014.8562651
TACCGGG5400.014.5979212
TAGACCG1108.6111383E-414.3148095
ACACGGG5750.014.1002293
CTGGGGG30450.013.7406065
CGACACG1555.83109E-513.06133121
Produced by FastQC (version 0.11.5)