FastQCFastQC Report
Wed 25 May 2016
SRR1700302_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700302_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6972391
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA183320.26292271905003606No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81660.11711907722903091No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA80560.11554142617647231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG77350.026.544331
TACCTGG77050.026.450322
ACCTGGG82950.023.8346183
CCTGGGG56950.019.3350454
TACCCGG6900.018.2765372
TATAACG5350.018.0996282
ATAACGC5350.017.6776923
TAACGCA5700.017.3823244
GTACACG5900.016.8079341
TACACGG5600.015.6830882
GTACCGG5250.015.4545691
GTACCCG8650.015.1121261
TCACGCA5000.013.5108064
TATCACG4700.013.4157552
TACCGGG5500.013.102142
CTGGGGG34400.012.9476575
GAGTACT144550.012.80755312
GTATAAC14100.012.7874681
GTATCAA244450.012.7694851
AGAGTAC219050.012.6417911
Produced by FastQC (version 0.11.5)