FastQCFastQC Report
Wed 25 May 2016
SRR1700301_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700301_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6196172
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA126940.2048684252147939No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62760.1012883438355165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG76550.027.2685411
TACCTGG76300.026.9813212
ACCTGGG81250.025.0580943
TATAACG5700.024.1044122
TAACGCA5900.022.903524
ATAACGC6500.022.8682823
GTACCCG7700.021.3711681
TACCCGG8100.020.0211522
GTACCGG5300.019.9902461
CCTGGGG52450.019.236924
GTACACG4850.018.59151
TACCGGG5750.017.6273172
TACACGG4950.016.8359682
CGACCGT1209.782727E-514.99647621
GTATAAC14300.014.9755821
CTGGGGG25550.014.1746375
TAACGGC2408.094503E-1014.05908236
CGCGGTA2154.091271E-813.60211543
GTACATG121350.012.9847491
TATACTG21350.012.9593795
Produced by FastQC (version 0.11.5)