FastQCFastQC Report
Wed 25 May 2016
SRR1700300_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700300_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9393255
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129000.13733258598856307No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97680.10398951162296777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA144750.018.859331
GTATCAA244800.018.2236391
GTACCTG68650.017.6990091
TACCTGG66550.017.6312392
ACCTGGG74300.015.2759923
TAACGCA5050.014.7160184
ATCAACG297600.014.5063093
TCAACGC299350.014.3989364
TATCAAC305600.014.2600422
CAACGCA303550.014.2208125
AACGCAG312500.013.8855116
TATAACG5600.013.6736382
ATAACGC4700.012.9373
GTACATG217000.012.8813891
AGAGTAC313900.012.65562711
GAGTACT199750.012.28626612
TATACTG35750.012.2698215
TACATGG217950.012.1828832
ACGCAGA353300.012.1738677
CCTATAC15650.012.0873933
Produced by FastQC (version 0.11.5)