FastQCFastQC Report
Wed 25 May 2016
SRR1700299_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700299_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7323819
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101450.13852062701167248No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76610.10460389586361978No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG63050.022.7405641
TACCTGG61750.022.4671632
GGTATCA109200.020.5413821
ACCTGGG65700.020.5325183
ATAACGC5400.020.4353983
TATAACG5650.019.1336252
TAACGCA6100.018.8287334
GTATCAA183950.017.8194311
CCTGGGG49500.015.9691824
ATCAACG220550.014.4793793
TCAACGC222700.014.3294784
GTACCCG7100.014.2883931
CAACGCA227100.014.0753535
TATCAAC229900.013.9794312
AACGCAG234150.013.75723656
TACCCGG7250.013.35780052
GTATAAC15200.013.3483671
GTACATG166900.012.7375111
AGAGTAC240450.012.451900511
TACATGG164450.012.2983532
Produced by FastQC (version 0.11.5)