FastQCFastQC Report
Wed 25 May 2016
SRR1700298_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700298_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7626374
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA146050.1915064747676943No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84660.11100950464794934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG69750.024.4083731
TACCTGG69600.024.0794322
ACCTGGG76900.021.1196023
TATAACG5300.019.9758172
ATAACGC5300.019.5505433
TAACGCA5450.018.5985284
GTACCGG5950.018.1911581
GTACACG5050.016.5213851
CCTGGGG55300.015.9262754
GTACCCG5850.014.2620511
TACCGGG5850.014.2471622
GTATTAG23050.014.1851251
GTATCAA223950.014.1569721
TACACGG5250.013.7300832
GTACATG144100.013.1916681
TATACTG27100.012.9494365
AGAGTAC221150.012.62360111
GAGTACT146250.012.59731212
TACATGG142600.012.5266862
GGTATCA155750.012.4944991
Produced by FastQC (version 0.11.5)