FastQCFastQC Report
Wed 25 May 2016
SRR1700297_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700297_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6150058
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA180940.2942086074635394No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88680.14419376207508938No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA78560.12773863270882974No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65700.10682826080664605No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG82650.028.3130532
GTACCTG83650.028.3004681
ACCTGGG89150.025.5413513
ATAACGC6200.024.3386883
TATAACG6150.022.7054772
GTACCCG8100.022.2676941
TAACGCA6750.021.6873234
CCTGGGG60500.021.4419124
TACCCGG8550.020.5467222
GTACCGG6650.018.6471021
TACCGGG6350.017.0247822
CTGGGGG28850.016.4527455
TACACGG4850.015.3244962
GTACACG4750.015.1889131
TAGACCG1402.2370079E-514.46159655
TCACGCA5800.013.20222954
GTACATG120850.012.8168221
TACACCG3950.012.5293165
ACGTCGC1801.6993277E-512.49767722
GTATAAC15000.012.4754761
Produced by FastQC (version 0.11.5)