FastQCFastQC Report
Wed 25 May 2016
SRR1700296_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700296_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5784891
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77610.1341598311878305No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60130.10394318579209184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG78050.029.1480481
TACCTGG79150.028.1451852
ACCTGGG81550.026.736153
TATAACG6350.024.1209262
TAACGCA6700.022.8598884
CCTGGGG53750.021.4970514
ATAACGC6950.021.3897953
GTACCGG6150.021.2639581
GTACCCG6600.020.4973891
GGTATCA73700.019.9773061
TACCGGG6150.019.7777752
GTATCAA128850.018.9686361
TACCCGG7300.018.5134412
GTACACG4750.017.088351
TACACGG4650.016.9540672
CCGACGC1803.925379E-916.24828332
CTGGGGG26650.015.868965
ATCAACG156100.015.2517453
TCAACGC157400.015.1255164
CGCGTAT1056.052082E-414.9967312
Produced by FastQC (version 0.11.5)