FastQCFastQC Report
Wed 25 May 2016
SRR1700295_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700295_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10142667
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123110.12137833175436008No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119300.023.7239761
GTATCAA226850.021.1949621
ACCGTCG2053.6379788E-1217.5572748
TACCTGG63000.016.7339442
ATCAACG281400.016.5464613
TCAACGC282200.016.4986574
CAACGCA285550.016.3229475
GTACCTG67550.016.225391
TATCAAC289200.016.2249742
AACGCAG294300.015.9599386
ACCTGGG68100.014.7859473
GTACATG256350.014.1109181
ACGCAGA335500.013.9463897
TACATGG255950.013.773752
GTACCGG6550.013.7721871
CGCAGAG340000.013.7022598
TATACTG37250.013.346185
AGAGTAC331250.013.17458211
ACATGGG257250.013.0998083
GAGTACT198200.013.07487912
Produced by FastQC (version 0.11.5)