FastQCFastQC Report
Wed 25 May 2016
SRR1700294_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700294_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6002994
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71560.11920718228270759No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG75000.028.079592
GTACCTG76250.028.0313341
ACCTGGG78600.025.9049453
TATAACG5000.022.9742092
ATAACGC5250.022.7380563
TAACGCA5200.020.7907944
CCTGGGG54100.020.316824
TACCGGG5650.019.1352082
GTACCGG5950.018.946471
GTATCAA141500.018.1007751
GTACCCG7250.018.037041
GGTATCA84800.017.9200551
GTACACG5500.017.6271061
TACACGG5200.016.4596522
TCGACGG850.00256935715.88003644
TACCCGG8200.015.6567442
ATCAACG167350.015.0067243
TCAACGC167750.014.997674
CAACGCA171500.014.6647665
TATCAAC177250.014.2322052
Produced by FastQC (version 0.11.5)