FastQCFastQC Report
Wed 25 May 2016
SRR1700293_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700293_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7011056
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA113630.16207258935030613No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81960.11690107738406312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG70750.026.2944981
TACCTGG71600.025.2595022
ACCTGGG75900.023.4723723
TATAACG4350.019.157322
CCTGGGG58300.018.9292894
ATAACGC4850.018.57553
GTACCGG7300.018.5338541
TAACGCA4950.016.379634
GTATCAA195850.015.5779951
TACCGGG7300.015.4265722
TACCCGG6050.015.2633832
GTACCCG6300.014.6750861
GTACACG6200.013.4569731
GGTATCA129400.013.4356121
GTACATG146200.013.4186631
TACACGG5950.013.2487032
CTGGGGG32750.013.1882895
AGAGTAC207950.013.11128711
TCAACGC227550.013.0945264
ATCAACG227600.013.0624293
Produced by FastQC (version 0.11.5)