FastQCFastQC Report
Wed 25 May 2016
SRR1700292_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700292_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11950001
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149440.1250543828406374No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG135700.026.3129541
TACCTGG134800.026.0511252
ATAACGC8850.024.4336973
ACCTGGG147850.023.3551163
TATAACG9450.022.1677422
TAACGCA10550.019.855834
CCTGGGG100800.019.418554
GTATCAA257100.018.7982311
GGTATCA149700.018.536621
GTACCCG12500.017.4926641
GTACCGG8300.017.1102661
TACCCGG13450.016.0775262
ATCAACG308500.015.3621423
TCAACGC310050.015.35786154
CAACGCA316050.015.0611875
TATCAAC321600.014.7857212
AACGCAG330300.014.5681766
GTACACG10350.014.1568711
TACACGG9700.013.9331662
TACCGGG9650.013.7719362
Produced by FastQC (version 0.11.5)