FastQCFastQC Report
Wed 25 May 2016
SRR1700291_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700291_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10538439
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA144950.13754408978407523No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124400.11804404807960647No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG99050.023.8515471
TACCTGG99700.023.2710172
ACCTGGG105300.021.4766373
ATAACGC6150.019.4116153
CGCGGTA4650.017.41745243
TATAACG6400.016.8940812
GTACACG9150.015.7677121
GTATCAA298300.015.7037021
CCTGGGG86500.015.5199034
TACCCGG10950.015.4283842
GTACCCG11950.015.0914791
GTACCGG8850.014.7738771
TAACGCA7100.014.5934634
GTATTAG28850.014.3774461
TTAACGG6250.013.6773435
GGTATCA195100.013.6459071
GTACATG249650.013.61694051
TAACGGC6150.013.53375936
ATCAACG350200.013.0247623
CGAACTA6050.013.0141229
Produced by FastQC (version 0.11.5)