FastQCFastQC Report
Wed 25 May 2016
SRR1700290_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700290_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15916062
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA167100.022.4907281
GTATCAA305450.020.0684321
ATCAACG361550.016.4599633
TCAACGC365950.016.2803694
CAACGCA373400.015.9167725
TATCAAC385200.015.6250512
AACGCAG383400.015.5957476
GTACATG364150.013.7441231
ACGCAGA445900.013.3646017
CGCAGAG450300.013.1890498
TACATGG359950.013.1728932
GTACCTG93700.013.0167351
TATACTG63600.013.016295
TACCTGG90250.012.7789162
GAGTACT252050.012.64712712
ACATGGG363250.012.54453853
AGAGTAC444900.012.52998111
GTATAGG33650.012.3945571
GTATTAG43550.012.3206381
GCAGAGT490700.011.965649
Produced by FastQC (version 0.11.5)