FastQCFastQC Report
Wed 25 May 2016
SRR1700289_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700289_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7938169
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89040.11216692413577993No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG104100.028.716221
TACCTGG103750.028.3538132
ACCTGGG113000.025.4343343
TATAACG6850.024.3331032
ATAACGC7100.023.158623
TAACGCA6950.022.361954
GGTATCA86500.020.9282991
CCTGGGG78550.020.158334
GTACCGG7450.019.9720291
GTATCAA153100.019.3635541
GTACCCG9650.018.2222421
TACCCGG9800.017.4680292
TACCGGG7450.016.9312152
ATCAACG189600.015.2418073
GTACACG7250.015.2367581
TCAACGC190450.015.1382064
CAACGCA194600.014.8312015
GTATAAC17800.014.8183681
CTGGGGG43300.014.8063995
TATCAAC198900.014.6426642
Produced by FastQC (version 0.11.5)