FastQCFastQC Report
Wed 25 May 2016
SRR1700288_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700288_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10321488
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA443930.4301027138722634No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA200160.19392552701703475No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT143450.13898189873398098No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT138880.13455424256657567No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC116400.11277443717417489No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG120250.028.3468231
TACCTGG118500.028.135212
ACCTGGG130150.025.1489473
CCTGGGG92450.019.9804524
TATAACG7100.018.7197692
TAACGCA6750.018.6888524
GTACCGG9300.018.6657521
TACCCGG9200.018.3645482
GTACCCG9900.017.7622151
ATAACGC8250.016.6561363
TACCGGG9700.015.792252
GTACATG206350.014.6726741
CTGGGGG50900.014.4959285
GTACACG8450.014.4070411
TACATGG203750.014.1078182
ACATGGG203550.013.6123283
TCTATAC25350.012.7073933
TCTGTCG15100.012.6632328
GTATTAA34200.012.39281
ACAACGC5850.012.322323
Produced by FastQC (version 0.11.5)