FastQCFastQC Report
Wed 25 May 2016
SRR1700287_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700287_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10065789
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150710.14972497436614257No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107160.10645961285300137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG125450.028.325561
TACCTGG124800.027.9701332
ACCTGGG135500.025.245693
TATAACG8200.024.9922282
ATAACGC8350.024.2730753
GGTATCA114800.022.9349371
TAACGCA9200.021.0500664
GTATCAA218700.020.8180941
TACCCGG10500.020.8046252
CCTGGGG88250.020.2604314
GTACCCG11050.020.21351
TACCGGG8600.018.592482
GTACCGG9800.017.726921
GTACACG7500.017.1467631
ATCAACG269200.016.429913
TCAACGC271700.016.3109154
CAACGCA276800.016.0670835
TATCAAC280750.015.8906672
AACGCAG288900.015.5336796
GTATAAC23450.015.4900061
Produced by FastQC (version 0.11.5)