FastQCFastQC Report
Wed 25 May 2016
SRR1700286_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700286_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11534978
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA150180.13019530683110103No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128020.11098417352854942No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG112550.023.649012
GTACCTG117100.023.541711
ACCTGGG121700.021.5002423
CCTGGGG110200.015.5694374
GTATCAA282750.014.88787651
GTACATG269650.014.6992451
TACATGG269650.014.1550122
GTACACG11700.013.6897721
GGTATCA190950.013.5981371
CCGACGC6000.013.49785232
TATAACG6400.013.3704072
CTATACT26450.013.2800124
TATACTG35950.013.2085515
ACATGGG277300.013.1388233
TACACGG11100.012.9836862
TCTATAC26200.012.7200323
TAGACTG21000.012.6454515
GTACCGT5000.012.6022586
TATACCG3950.012.5342165
TACACCG7200.012.5025645
Produced by FastQC (version 0.11.5)