FastQCFastQC Report
Wed 25 May 2016
SRR1700285_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700285_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12596821
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167030.1325969464835612No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA135750.024.4166411
GTACCTG121250.023.6333871
TACCTGG120100.023.2504352
GTATCAA262850.021.1598661
ACCTGGG128200.021.1133333
GTACACG9200.017.1677821
ATCAACG325200.016.7157963
TCAACGC328000.016.5858424
CCTGGGG97350.016.3992774
CAACGCA333750.016.3119745
TATCAAC342750.015.9983022
AACGCAG343700.015.9376516
GTACCGG10800.015.6690071
TAACGCA9500.013.9843684
ATAACGC9200.013.9517083
ACGCAGA394000.013.84046657
TACCGGG11350.013.6900682
CCGACGC3950.013.66852332
CGCAGAG400400.013.5742918
TACACGG10350.013.4897462
Produced by FastQC (version 0.11.5)