FastQCFastQC Report
Wed 25 May 2016
SRR1700284_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700284_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12433733
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA420890.3385065450577071No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA191810.15426581864030697No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT136250.1095809279481874No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133960.10773916409496649No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT129320.1040073805670429No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG129650.025.8115122
GTACCTG131350.025.8014141
ACCTGGG138800.023.5090753
CCTGGGG112050.017.9461694
ATAACGC9300.017.6765123
TATAACG8950.017.3617362
TAACGCA8850.017.302064
GTACCCG14600.015.1452651
TACCCGG14550.014.3941852
GTACATG273100.014.1361761
TACATGG273200.013.4278812
GTACCGG12550.013.12451
ACATGGG270550.012.9181643
GTATTAA40800.012.60893251
CTGGGGG66650.012.088545
GTATTAG28850.012.0442171
GTATATA48950.011.9385261
GTACACG10050.011.8990811
AGAGTAC337800.011.77421411
GAGTACT208200.011.680119512
Produced by FastQC (version 0.11.5)