FastQCFastQC Report
Wed 25 May 2016
SRR1700283_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700283_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8963353
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA106480.11879483046132401No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94060.10493840865131608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG91950.023.5241831
TACCTGG92700.022.7846682
ACCTGGG93450.022.1915593
TATAACG6300.017.1561682
ATAACGC7450.016.3209343
GTATCAA213550.015.92756751
CCTGGGG79850.015.9032164
GTACACG8400.015.8423811
TAACGCA6550.015.8123834
GGTATCA141050.014.7276761
TACACGG8550.014.4849222
GTACCGG7900.014.2754761
GTACCCG9100.014.12801551
GTACATG205250.013.7913961
TACATGG202050.013.3956952
TATACTG29450.012.7616955
ATCAACG258050.012.7134443
TCAACGC259900.012.6828154
ACATGGG204200.012.6588333
TACACCG6050.012.6473845
Produced by FastQC (version 0.11.5)