FastQCFastQC Report
Wed 25 May 2016
SRR1700282_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700282_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14329351
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG114850.022.3744722
GTACCTG119000.022.2231921
ACCTGGG124250.020.0467853
GTATCAA305050.016.9834791
GGTATCA185500.016.7013841
TATAACG8500.015.3676412
CCTGGGG94200.015.1321594
ATCAACG362700.013.9397453
CGCGGTA5650.013.93538243
TAACGCA7600.013.9261914
ATAACGC10050.013.8934993
TCAACGC364400.013.8735024
GTACATG304000.013.8652941
GTATTAG44300.013.7526331
CAACGCA369750.013.6714985
AACGCAG379600.013.45278556
GTACACG10650.013.3480171
GTACCGG10400.013.234951
TATCAAC386600.013.2065022
TACATGG304550.013.0744022
Produced by FastQC (version 0.11.5)