FastQCFastQC Report
Wed 25 May 2016
SRR1700281_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700281_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9699781
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140550.14490017867413707No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105500.10876534222782969No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG126250.027.8210241
TACCTGG125900.027.3939442
ACCTGGG134100.025.2310353
ATAACGC7950.021.5347793
CCTGGGG100200.020.795434
TAACGCA8600.019.9071484
GGTATCA131750.019.5925921
GTACACG8850.019.360091
TATAACG8450.019.1946812
GTATCAA230650.019.1184391
GTACCCG12350.017.1592351
TACCCGG12650.016.3833392
TACACGG10600.016.15152
ATCAACG276800.015.4625623
TCAACGC279150.015.324324
CAACGCA284700.015.0678845
TATCAAC290250.014.9249262
AACGCAG296500.014.7113776
GTACCGG10500.014.6001271
GTATAAC22300.013.951191
Produced by FastQC (version 0.11.5)