FastQCFastQC Report
Wed 25 May 2016
SRR1700280_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700280_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8725123
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA316140.3623330009215916No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA131980.15126434320753987No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121500.13925305121773068No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93430.10708158498166731No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT92640.10617615361984008No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT89710.1028180347715442No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG87050.025.087331
TACCTGG87250.024.728652
ACCTGGG97500.021.5276553
ATAACGC6000.021.0194913
TAACGCA6500.019.0559144
TATAACG6300.018.9467932
CCTGGGG67500.017.6828614
GTACCGG7050.016.942841
GTACACG6950.015.2409671
GTACCCG7400.014.9232641
TACCGGG7250.014.6002462
CGCGGTA3700.013.98518643
TACCCGG8050.013.4290632
TATACTG26800.013.1786125
GTACATG168850.012.8135551
CGCAACG1607.846672E-512.65398617
CTGGGGG35750.012.4593085
TACACGG7600.012.4461792
GAGTACT207350.012.35746812
AGAGTAC295000.012.30810811
Produced by FastQC (version 0.11.5)