FastQCFastQC Report
Wed 25 May 2016
SRR1700279_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700279_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6404087
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA234050.3654697383093015No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA91690.14317419485400495No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72890.11381794157387305No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT69250.10813407125793263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG69900.025.6950421
TACCTGG70250.024.8115462
TATAACG5850.024.2517952
ATAACGC6000.024.020453
TAACGCA5800.022.9074334
ACCTGGG76950.022.0655693
GTACCGG5550.020.7083441
CCTGGGG50450.018.1224654
TACCGGG5350.017.2579522
TACCCGG5750.017.232332
GTACCCG6050.016.7619821
GTACACG4250.015.9074481
TACACGG4350.013.9776352
GTATAAC15050.013.6261471
CGAACGC1555.825545E-513.06268230
TCTGTCG8700.012.9289168
TACCGTC2104.526155E-712.8550377
TATACTG23250.012.4817295
CTGGGGG26750.012.2782675
TATCACG4900.011.9491352
Produced by FastQC (version 0.11.5)