FastQCFastQC Report
Wed 25 May 2016
SRR1700278_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700278_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8847078
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA201880.22818833517687986No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA97130.1097876609655753No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94760.1071088103891477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG90000.025.947941
TACCTGG90350.025.4773562
ACCTGGG98150.022.6489773
TATAACG5850.017.7106132
GTACCGG7100.017.461841
TACCGGG6850.017.4267712
ATAACGC5950.017.4125613
CCTGGGG67100.017.051424
CGCGTAT800.001714410716.87155214
TACCCGG8050.016.7875392
GTACCCG8550.016.345991
GTACACG7350.015.6411431
TATACCG4100.015.3627145
TACACGG7850.014.0591172
GTACATG173850.013.7700491
TAGACCG1801.1272969E-613.747195
TACATGG171750.013.2320172
TAACGCA7450.012.9996464
GTATCAA276650.012.89841
ACATGGG168500.012.7784973
Produced by FastQC (version 0.11.5)