FastQCFastQC Report
Wed 25 May 2016
SRR1700276_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700276_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8932240
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126180.1412635576294412No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92820.10391570311590373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG111800.028.9991531
TACCTGG111600.028.5840592
ACCTGGG121900.025.5954993
TATAACG7400.022.8325482
ATAACGC7750.022.6730753
TAACGCA7450.021.7715264
CCTGGGG80150.021.3329494
GTACCCG7700.020.7891081
TACCCGG8700.017.8671252
GTATCAA217050.017.4197351
GTACCGG8350.017.2807161
TACCGGG8050.016.7911422
GGTATCA131600.016.6182041
GTACACG6100.015.8930371
TCTGTCG15600.015.7180648
CTGGGGG43700.015.0825095
CTGTCGC17100.014.7339459
ATCAACG260550.014.0933443
GTACATG165700.013.987461
TCAACGC262850.013.9527264
Produced by FastQC (version 0.11.5)