FastQCFastQC Report
Wed 25 May 2016
SRR1700275_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700275_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10669536
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA243300.2280324092819032No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153890.14423307630247464No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111480.10448439369809522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG115200.026.7324181
TACCTGG117050.025.9711062
ACCTGGG121550.024.2307383
ATAACGC8550.019.7519053
TATAACG7800.019.6312
CCTGGGG96550.019.3333874
TAACGCA8400.018.4959034
GTACCGG9850.015.346051
TACCCGG10300.013.9917122
GTATCAA352550.013.9442311
GTACATG239900.013.880791
TACGACG2855.4569682E-1113.4250625
GTATTAG26100.013.3982851
TACATGG238500.013.208622
CTGGGGG54700.013.2077845
GTACCCG11200.013.0934281
TACCGGG8750.012.8673782
ACGTCGC3001.2914825E-1012.74765322
TATACCG4250.012.7096795
TATACTG41000.012.6806165
Produced by FastQC (version 0.11.5)