FastQCFastQC Report
Wed 25 May 2016
SRR1700274_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700274_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8886992
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124820.1404524725576438No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93660.10538999022391378No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG103700.028.0422461
TACCTGG103150.027.8152072
ACCTGGG109100.025.7609653
TATAACG7000.021.5555382
ATAACGC7350.020.8350183
CCTGGGG82750.020.4910894
TAACGCA7450.019.0424294
GGTATCA121650.018.6191651
GTATCAA209100.018.4924931
CGCGTAA850.002569645915.879889510
GTACCGG8000.015.7919831
GTACACG8000.015.7919831
GTACCCG8600.015.2148671
ATCAACG253400.014.8594243
TCAACGC254350.014.8470234
CAACGCA258750.014.6128425
TATCAAC262200.014.4555142
CTGGGGG44800.014.3176785
AACGCAG266750.014.3005896
GTACATG186150.013.9856091
Produced by FastQC (version 0.11.5)