FastQCFastQC Report
Wed 25 May 2016
SRR1700273_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700273_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10338977
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131710.12739171389974077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG118100.027.789611
TACCTGG122350.026.5390762
ACCTGGG129450.024.386973
GGTATCA114400.023.0098721
TATAACG6500.020.092742
GTATCAA203200.019.5037171
CCTGGGG105150.018.6512584
TAACGCA7400.017.9522784
GTACCGG8800.017.9425741
GTACACG9900.017.543851
ATAACGC8150.017.4058343
TACCGGG9250.015.8232652
ATCAACG244800.015.7380333
TCAACGC245550.015.6986794
CAACGCA249600.015.4642785
TATCAAC257800.015.1108082
TACACGG10300.015.0846892
AACGCAG259250.014.9839346
GTACCCG10050.014.8131441
GTACATG230250.014.3714251
Produced by FastQC (version 0.11.5)