FastQCFastQC Report
Wed 25 May 2016
SRR1700272_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700272_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8267467
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108780.13157597121343212No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83090.10050236668619301No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG123450.030.265561
TACCTGG124100.029.4876352
ACCTGGG129300.027.7434083
TATAACG8100.025.577682
ATAACGC8100.024.186713
TAACGCA7600.023.9988464
CCTGGGG91750.022.8487554
GTACCCG9750.021.9835821
GGTATCA88300.021.667791
GTATCAA161700.020.4551851
TACCCGG10500.019.302412
GTACACG7350.018.7250061
TACCGGG6950.018.4692132
GTACCGG8150.018.2711471
CTGGGGG46000.017.467035
GTATAAC18200.016.4876881
TACACGG8400.016.3534322
ATCAACG199200.016.176813
TCAACGC201050.016.0942824
CAACGCA203950.015.8798265
Produced by FastQC (version 0.11.5)