FastQCFastQC Report
Wed 25 May 2016
SRR1700271_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700271_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5338897
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA225840.42300872258820504No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA95210.1783327155403073No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT74620.13976669712863912No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT59600.1116335452809822No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC54710.10247435003896872No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54450.10198735806291075No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG84200.030.408062
GTACCTG85350.030.267581
ACCTGGG88550.028.252553
TATAACG5300.025.4926912
ATAACGC5500.024.9746443
TAACGCA5400.023.7686824
CCTGGGG59150.023.602664
GTACACG5650.020.3656141
TACACGG5750.018.7980882
GTACCGG6050.018.64621
TACCGGG6050.017.4937442
GTATAAC10800.017.3392411
TACCCGG6550.017.1897282
GTACCCG6650.016.9638391
TACGTCG1552.0748121E-715.96458821
CTGGGGG30850.015.3201945
GACGCTA2850.014.99703840
ACACGGG6650.014.8992093
GTACATG100650.014.2342861
CGCAACG1758.2029146E-714.14006417
Produced by FastQC (version 0.11.5)